| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 181 | Thiobacillus denitrificans | TCCAGGATTACCAGCGTGC | TTTCGAGGTTGGTGCGCT | 59.78 | 59.89 | 195 | 84 |
100.00%
|
100.00%
|
| Primer 182 | Thiobacillus denitrificans | AGCGCAAATCGTCCAGGAT | TTTCGAGGTTGGTGCGCT | 59.78 | 59.89 | 206 | 84 |
100.00%
|
100.00%
|
| Primer 183 | Thiobacillus denitrificans | TTCAGCGCAAGGATGGCA | TTCGGCAGCGCTTCGAA | 59.97 | 60.01 | 220 | 84 |
100.00%
|
100.00%
|
| Primer 184 | Thiobacillus denitrificans | GCAAGGAAATGCTGCGCT | CAAACGCGTTCTGCAGCA | 59.43 | 59.67 | 189 | 84 |
100.00%
|
100.00%
|
| Primer 185 | Thiobacillus denitrificans | GCAAGGAAATGCTGCGCT | TCGACGAGCTGTTGGCAA | 59.43 | 59.58 | 204 | 84 |
100.00%
|
100.00%
|
| Primer 186 | Thiobacillus denitrificans | CGCTGATCTTCCTGTCGCT | AGTACGCGAGCATCAGCA | 59.86 | 59.11 | 165 | 84 |
100.00%
|
100.00%
|
| Primer 187 | Thiobacillus denitrificans | GCTGAAAAGCCACGGCAT | AACGTCGACGAGCTTGCT | 59.04 | 59.66 | 199 | 84 |
100.00%
|
100.00%
|
| Primer 188 | Thiobacillus denitrificans | AAGCCACGGCATACAGGT | AACGTCGACGAGCTTGCT | 58.92 | 59.66 | 193 | 84 |
100.00%
|
100.00%
|
| Primer 189 | Thiobacillus denitrificans | TTCGCTGCGCTGATGGAA | GCCTTCTGCAGACCGATGT | 60.05 | 60.08 | 293 | 84 |
100.00%
|
100.00%
|
| Primer 190 | Thiobacillus denitrificans | TTCGCTGCGCTGATGGAA | TCTGCAGACCGATGTCGTC | 60.05 | 59.49 | 289 | 84 |
100.00%
|
100.00%
|